Journal: BBA Clinical

Article Title: Transferrin-bound proteins as potential biomarkers for advanced breast cancer patients

doi: 10.1016/j.bbacli.2014.08.004

Figure Lengend Snippet: List of proteins found to be differentially expressed at statistically significant levels (p ≤ 0.05, fold change ≥ 2) when comparing Transferrin-associated proteins in normal control (n = 10) to stage IV breast cancer (n = 10) patient serum samples (Discovery sample set). Included in the table is the gene symbol count, c, protein identification, peptide confidence score (Mascot), p-value, fold-change and highest/lowest mean change.

Article Snippet: ELISA kits for Fibrinogen (EF1040-1) and for Fibronectin (EF1045-1) from Assaypro were used as per manufacturer's instructions (AssayPro, Winfield, MO, USA).

Techniques: Control

Journal: BBA Clinical

Article Title: Transferrin-bound proteins as potential biomarkers for advanced breast cancer patients

doi: 10.1016/j.bbacli.2014.08.004

Figure Lengend Snippet: Box and whisker plots (range, median, quartiles) of A. Fibronectin, B. Fibrinogen and C. CA15-3 in control, IBD (Inflammatory Bowel Disease), benign breast disease and stages I–IV breast cancer patient serum samples (validation sample set). Fibrinogen, Fibronectin and CA15-3 were all found to be statistically significant when comparing abundance levels between normal control and stage IV breast cancer patient serum samples. Both Fibrinogen and CA15-3 were also statistically significant comparing IBD or benign breast disease to stage IV breast cancer. Samples: normal healthy control (n = 54), inflammatory bowel disease (n = 19), benign breast disease (n = 12), stage I breast cancer (n = 20), stage II breast cancer (n = 20), stage III breast cancer (n = 39) and stage IV breast cancer (n = 76).

Article Snippet: ELISA kits for Fibrinogen (EF1040-1) and for Fibronectin (EF1045-1) from Assaypro were used as per manufacturer's instructions (AssayPro, Winfield, MO, USA).

Techniques: Whisker Assay, Control, Biomarker Discovery

Journal: Molecules

Article Title: Vincamine Ameliorates Epithelial-Mesenchymal Transition in Bleomycin-Induced Pulmonary Fibrosis in Rats; Targeting TGF-β/MAPK/Snai1 Pathway

doi: 10.3390/molecules28124665

Figure Lengend Snippet: Effect of vincamine on fibronectin ( A ), N-cadherin ( B ), and collagen ( C ) levels in lung tissues. Bars represent mean ± SD. Significant difference was analyzed by one-way ANOVA test followed by post hoc Dunnett test, where * p < 0.001, compared to sham group, and # p < 0.001, compared to BLM-induced group.

Article Snippet: In lung tissue homogenates, levels of fibronectin, N-cadherin, and collagen were also assessed, according to the manufacturer’s instructions, utilizing rat fibronectin ELISA kit (#MBS761397, MyBioSource, CA, USA), N-cadherin ELISA kit (#KOA0665, Rockland immunochemical Inc., Royersford, PA, USA), and collagen type I ELISA kit (#MBS262647, MyBioSource, CA, USA), respectively.

Techniques:

Journal: Journal of Translational Medicine

Article Title: LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation

doi: 10.1186/s12967-020-02310-2

Figure Lengend Snippet: LTBP1 contributed to CAFs transformation and expression of FN1 induced by ESCC cells. a The expression of LTBP1 and FN1 were positively correlated in the GEPIA database (Spearman correlation analysis, r = 0.6, p < 0.001). b , c The expression of LTBP1 and FN1 were detected in various cells by western blot and ELISA. d , f Western blot and immunofluorescent analysis of α-SMA and FN1 expression in fibroblasts which were con-cultured with si-NC, si-LTBP1 ESCC cells or negative for 96 h. e CCK8 assays comparing the effect of (± siLTBP1 ESCC cells or negative) conditioned medium on the activity of the fibroblasts. Each experiment was performed in triplicate. Paired-samples t-test, *p < 0.05

Article Snippet: The levels of FN1 in conditioned medium of various cells were measured using Human Fibronectin ELISA Kit (CSB-E04551h; CUSABIO) according to the manufacturer’s instructions.

Techniques: Transformation Assay, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture, Activity Assay

Journal: Chinese Medical Journal

Article Title: Exosomal miR-485-3p derived from pancreatic ductal epithelial cells inhibits pancreatic cancer metastasis through targeting PAK1

doi: 10.1097/cm9.0000000000002154

Figure Lengend Snippet: Figure 2: MiR-485-3p regulated PC cell migration and Invasion. (A, B) miR-485-3p was overexpressed and silenced In BxPC-3 and PANC-1 cells. (C, D) Transwell migration and Invasion assays in BxPC-3 and PANC-1 cells with overexpression or silencing of miR-485-3p (bar: 100 mm). The migrated or invaded cells were imaged at 200 magnification in five random fields and counted using ImageJ software. (E) MiR-485-3p was overexpressed and silenced in PSCs. (F) FN1, COLIA1, and FAP levels in cell supernatant of PSCs transfected with miR-485-3p mimic or Inh-miR-485-3p were assessed by ELISA. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. COLIA1: Collagen type alpha 1 chain; ELISA: Enzyme linked immunosorbent assay; FAP: Fibroblast activation protein alpha; FN1: Fibronectin 1; PC: Pancreatic cancer; PSCs: Pancreatic stellate cells.

Article Snippet: Enzyme linked immunosorbent assay (ELISA) The levels of fibronectin 1 (FN1), Col I, and fibroblast activation protein alpha (FAP) in PSCs cell supernatant were detected by using Human FN ELISA Kit (No. E-ELH0179c, Elabscience, Wuhan, China), Human Col I ELISAKit (No. JM-03329H1, JINGMEI, Jiangsu, China), and Human FAPa ELISA Kit (No. EH3037, FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Migration, Over Expression, Software, Transfection, Enzyme-linked Immunosorbent Assay, Activation Assay

Journal: Advanced Science

Article Title: Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

doi: 10.1002/advs.202510631

Figure Lengend Snippet: Co‐culture experiments demonstrate that Fn1 derived from Trem2⁺ macrophages activates fibroblasts via Itga8. A) Schematic representation of the co‐culture experiment design. B) Flow cytometry confirms that IL‐17a induces the differentiation of BMDM into Trem2⁺ macrophages in vitro. C) Quantification of the percentage of Trem2 + macrophage in (B), n = 3 per group. D) Representative Western blot images of in vitro‐induced Trem2⁺ macrophages. E) ELISA analysis of Fn1 expression in the conditioned medium. F) Representative Western blot images of primary bladder fibroblasts treated with conditioned medium from IL‐17a‐induced BMDM. G,H) qRT‐PCR (G) and Western blot (H) analysis of Fn1 expression at the mRNA and protein levels following transfection with Fn1 siRNA. I) ELISA analysis of Fn1 expression in the culture supernatant following transfection with Fn1 siRNA. J) Representative Western blot images of fibroblasts treated with conditioned medium from IL‐17a‐induced BMDM transfected with either si‐NC or si‐Fn1. K) Representative images of the contraction assay in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with the conditioned medium. L) Quantification of cell contraction in (K), n = 4 per group. M) Representative Western blot images of fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with conditioned medium from IL‐17a‐induced BMDM. N) Representative images of the scratch assay in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with Fn1. O) Statistical analysis of the wound area over time in (N), n = 3 per group. P) Representative images of immunofluorescence staining in fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with Fn1. Q) Quantification of α‐SMA/F‐actin in (P), n = 3 per group. R,S) qRT‐PCR (R) and Western blot (S) analysis of Trem2 expression at the mRNA and protein levels following transfection with OE‐Trem2. T) Flow cytometry confirms that IL‐17a induces the differentiation of BMDM into Trem2⁺ macrophages in vitro. U) Quantification of the percentage of Trem2 + macrophage in (T), n = 3 per group. V) Representative Western blot images of fibroblasts transduced with sh‐NC or sh‐Itga8 lentivirus and treated with conditioned medium from Trem2‐overexpressing BMDM. Mφ = Macrophage. Data represent mean ± SD. Unpaired two‐tailed t‐test was used for C, E, G, I, R, and U. One‐way ANOVA was used for L, and Q. Two‐way ANOVA was used for O. * P < 0.05, ** P < 0.01, *** p < 0.001. Figure was created in BioRender. Wang, J. (2025) https://BioRender.com/kp68v7m .

Article Snippet: The concentration of Fn1 in the supernatant of macrophage culture medium was determined using an ELISA kit (EK0350, Boster, Wuhan, China).

Techniques: Co-Culture Assay, Derivative Assay, Flow Cytometry, In Vitro, Western Blot, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Transfection, Contraction Assay, Transduction, Wound Healing Assay, Immunofluorescence, Staining, Two Tailed Test

Journal: Cancers

Article Title: ROCK2 Confers Acquired Gemcitabine Resistance in Pancreatic Cancer Cells by Upregulating Transcription Factor ZEB1

doi: 10.3390/cancers11121881

Figure Lengend Snippet: Primer for PCR assay.

Article Snippet: Fibronectin was purchased from Proteintech Group Inc. P-ROCK1 (Thr455 + Ser456) was purchased from Bioss (Beijing, China).

Techniques: